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Job Title: Molecular and Cellular Biologist Location: Cambridge, MA 02139 Duration: 6 months contract Pay Range: $40-46/hour on W2 Job Description: POSITION SUMMARY: We are seeking a scientist to join our Systems Immunology Lab in clent's Inflammation & Immunology Research Unit. The successful candidate will be responsible for generating massive single-cell perturbational data using functional genomics and single cell transcriptomics technologies in collaboration with a small team. POSITION RESPONSIBILITIES: Generate custom lentiviral libraries and maintain extensive cell cultures. Generate edited pools of cells via lentiviral transduction and arrayed electroporation. Generate single-cell data via several commercial workflows, including from 10x Genomics. Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale. ORGANIZATIONAL RELATIONSHIPS: Reports to a lead scientist in Systems Immunology Lab. Collaborates with and supports several multi-disciplinary experimental teams on a per-project basis. EDUCATION AND EXPERIENCE: Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field TECHNICAL SKILLS REQUIREMENTS: 3+ years of hands-on experience with standard cell culture techniques including primary immune cells handling and isolation, maintenance of cell lines and in vitro stimulation of primary immune cells, experience with standard molecular biology techniques including lentivirus infection, qPCR, western blot, and flow cytometry. Bonus: direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing. PHYSICAL POSITION REQUIREMENTS: • Typical bench work, including sitting, pipetting, lifting <10lb items Hard Skills: 1. Diverse Cell Types 2. Molecular biology techniques *qPCR 3. Gene Editing Preferred: Direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing. Interview Process: 1st Teams screen (Camera-On) 2nd Onsite (2hr with Stakeholders)